IL-2 Detection (CAT#: STEM-MB-0271-WXH)

Introduction

IL-2 is a cell growth factor in the immune system produced by activated T lymphocytes, also known as T cell growth factor. IL-2 can activate T cells, participate in the induction of Cytotoxic T lymphocyte production. Besides, it can cause proliferation of B cells and stimulate NK cell production, proliferation and activation. The cell proliferation response induced by it is a key factor affecting the intensity of the immune response. IL-2 receptor IL-2R is composed of three chains, α, β and γ, respectively, while IL-2 can induce IL-2Rα expression and induce phosphorylation of Jak1 and Jak3, among which Jak1 and Jak3 can Binds to IL-2β and γ. At present, IL-2 can be used for immunotherapy of tumors, and can also be used as an immunoadjuvant to enhance the effect of conventional vaccine immunization. It reduces the side effects of vaccine immunization, and also treats infectious diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

IL-2 must bind to the receptor to exert biological effects. However, IL-2Rα is rarely expressed in resting T cells. But when T cell receptor is activated, IL-2Rα expression is increased, so IL-2Rα is a marker of T cell activation. IL-2Rβ plays a major role in mediating signal transduction. It activates MAPK and PI3K adaptor protein Shc in the MAPK pathway, ultimately activate the MAPK pathway. In the JAK/STAT5 pathway, the combination of β and STAT factor make STAT5 phosphorylate, thereby enabling up-regulation of target gene expression. IL-2Rγ, like β, is also capable of signal transduction. IL-2, through complex regulation, can make the signal path perform correctly.

Applications

IL-2 can exert immunoregulatory functions.
IL-2 can activate T cells, participate in the induction of Cytotoxic T lymphocyte production.
IL-2 can cause proliferation of B cells and stimulate NK cell production, proliferation and activation.
IL-2 is involved in the inflammatory response, maintaining immune system homeostasis and anti-tumor effects.

Procedure

1. Process samples.
2. IL-2 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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