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Immunofluorescence technique, also known as fluorescent antibody technique, is one of the earliest developed labeling immunology techniques. It is a technique established on the basis of immunology, biochemistry and microscopy. The main features of this technique are: strong specificity, high sensitivity and fast speed.
The basic reaction in immunology is the antigen-antibody reaction. Because the antigen-antibody reaction is highly specific, when an antigen-antibody reaction occurs, as long as one of the factors is known, the other factor can be detected. Immunofluorescence technique is to label the antibody (or antigen) with a fluorescent dye that does not affect the activity of the antigen and antibody, and after binding to the corresponding antigen (or antibody), a specific fluorescent reaction is presented under a fluorescent microscope.