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Immunofluorescence Technique (CAT#: STEM-PET-0003-WXH)

Introduction

Immunofluorescence technique, also known as fluorescent antibody technique, is one of the earliest developed labeling immunology techniques. It is a technique established on the basis of immunology, biochemistry and microscopy. The main features of this technique are: strong specificity, high sensitivity and fast speed.
The basic reaction in immunology is the antigen-antibody reaction. Because the antigen-antibody reaction is highly specific, when an antigen-antibody reaction occurs, as long as one of the factors is known, the other factor can be detected. Immunofluorescence technique is to label the antibody (or antigen) with a fluorescent dye that does not affect the activity of the antigen and antibody, and after binding to the corresponding antigen (or antibody), a specific fluorescent reaction is presented under a fluorescent microscope.




Applications

• Analyze the distribution of proteins, glycans, and small biological and non-biological molecules
• Detect particular proteins
• Evaluate cultured cells
• Study DNA sequences on chromosomes

Procedure

1. Cell Preparation
2. Fixed
3. The cell membrane is permeable to ensure that the antibody can reach the antigen site
4. closed
5. Primary antibody binding
6. Secondary antibody binding
7. Mounting and testing

Notes

Customer provides sample and primary antibody for detection.
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