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LncRNA Sequencing (CAT#: STEM-MB-0044-WXH)

Introduction

Long non-coding RNAs (long ncRNAs, lncRNA) are a type of RNA, generally defined as transcripts more than 200 nucleotides that are not translated into protein.
lncRNA are involved in a wide range of important cellular processes and play a critical role in human diseases such as cancer, cardiovascular disease and neurological disorders. lncRNA-Seq reveals the complete picture of the lncRNA in a biological sample under specific conditions.
The technical principle is to eliminate the rRNA with the highest content in the RNA, and then filter out the small RNAs with high content such as tRNA and snRNA through magnetic bead screening, and then build a library and sequence all the remaining RNA.
Compared with the common transcript library method, the accuracy of lncRNA sequencing is better, and more abundant information can be obtained.




Applications

• Epigenetic research
• Gene function research
• ceRNA network research
• Gene regulation research
• Cancer and marker research
• Drug target research

Procedure

1. Quality inspection of total RNA samples
2. Strand-specific library construction, library detection and sequence
3. Data quality control
4. Reference sequence alignment
5. mRNA analysis, InRNA analysis

Notes

Clients provide qualified RNA or tissue cell samples.

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