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Neurotransmitter is a kind of special chemical substance that is released from the presynaptic membrane during the chemical synaptic transmission of neurons and transmits information to the postsynaptic membrane. Neurotransmitters are mainly divided into biogenic amines, peptides, amino acids and others according to their structures. Neurotransmitters and their metabolites are widely distributed in the central and peripheral nervous systems, and play a very important role in maintaining various life activities. Therefore, high-throughput detection and analysis of neurotransmitters has important research significance for revealing the pathogenesis, diagnosis and treatment of nervous system diseases.