Analysis of Xanthine Oxidase (XOD) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1503-LGZ)

Introduction

Sensitivity: 0.01 U/L
Detection range: 0.01 -1.2 U/L
Precision: inter-lot difference of 9%, intra-lot difference of 4.1%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Hypoxanthine can be oxidized by xanthine oxidase (XOD) to produce xanthine and superoxide anion, which is quickly converted into hydrogen peroxide in the system. Under the action of peroxidase, hydrogen peroxide oxidizes the non-fluorescent probe into a fluorescent substance. By measuring the fluorescence value of the system, the activity of the corresponding xanthine oxidase can be calculated.

Applications

It is suitable for the determination of xanthine oxidase activity in serum, plasma and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma and animal tissue samples

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