Crosslinking-immunprecipitation Sequencing (CLIP-seq) (CAT#: STEM-MB-0072-WXH)

Introduction

Crosslinking-immunprecipitation sequencing (CLIP-seq) is an antibody-based technology used to investigate RNA-protein interactions in RNA immunoprecipitation. The technology uses specific antibodies of RNA-binding proteins to precipitate RNA-protein complexes and retrieve RNA fragments for high-throughput sequencing, uncovering in-depth the regulatory effects of RNA-binding proteins and RNA molecules and their significance to life.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Confirm the direct interaction of RNA with the target protein as well as the exact binding sites
• Identify RNA-RBP interaction networks across the entire genome
• Identify and study the functional mechanisms of lncRNA, circRNA, and miRNA

Procedure

1.Preparation of cross-linked cell lysate
2.Purification of specific cross-linked RNA fragments
3.cDNA library sequencing and analysis

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