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Lipid Metabolism Pathway Assay (CAT#: STEM-MB-0365-WXH)

Introduction

Lipids are a large class of compounds that occur in nature and are heterogeneous in chemical composition and structure. The structural diversity of lipids endows them with a variety of important biological functions. Lipids are the main components of biological membranes. They are involved in signaling, regulation of cell growth, proliferation, differentiation, senescence, programmed death, survival, apoptosis, inflammation, motility, membrane homeostasis, chemotherapeutic response and drug resistance. In addition, they provide energy and are involved in the growth and maintenance of life activities of organisms.
Lipid metabolic transduction studies include: lipid and metabolite analysis and identification; lipid function and metabolic regulation (including key genes/proteins/enzymes); lipid metabolic pathways and networks.




Principle

Lipid metabolism mainly includes triglyceride (TG) metabolism, metabolism of cholesterol and its esters, and phospholipid and glycolipid metabolism. In these metabolic processes, many proteases, receptors, transcription factors, etc. are involved, and they are regulated by some signal transduction pathways, forming a complex and fine regulatory network to maintain the lipid metabolism balance of cells and the whole body. Lipid metabolism transduction signal pathways mainly include peroxisome proliferator-activated receptor (PPAR) signal transduction pathway, liver X receptor (LXRs) signal transduction pathway, and sterol regulatory element-binding protein (SREBPs) signal transduction guide route.

Applications

Study the regulatory mechanism of lipid metabolism signaling pathway in clinical medicine
Research on the influence of various medical viruses on lipid metabolism signaling pathway
Study the effects of carcinogenic drugs or carcinogenic therapies on lipid metabolism signaling pathway

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: IPS-1, ISGF3, MSK2, PI3K, TBK1, Vav, ATG13, ATG2, ATG12, ATG5, ATG7, ATG10, ATG3, ATG4, JNK, MYD88, Rac1, TLR4, ATG16, WIPi2, LC3I, PROLC3, ULK, FIP200, Histone-H3, IRF5, MEK3, p38MAPK, SH2, TRAF6, VPS15, VPS34, UYRAG, NFκB, RIG-1, TRAF3, GAS, IRF9, AMBRA1, p38, RIP1, TRAF5, IRAK1, ISRE, mTOR, PKR, TLR3

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