Analysis of NADP-Malate Dehydrogenase (NADP-MDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1465-LGZ)

Introduction

Sensitivity: 0.63 U/L
Detection range: 0.63–50.0 U/L
Precision: inter-lot difference of 6.4%, intra-lot difference of 1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 440-460 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

According to different coenzyme specificities, MDH is divided into NAD-dependent MDH and NADP-dependent MDH, and NADP-MDH mainly exists in eukaryotic cells. NADP-malate dehydrogenase catalyzes the conversion of malic acid and NADP+ into oxaloacetate and NADPH, and NADPH makes WST-8 orange-yellow under the action of electron coupler. Malate dehydrogenase activity can be measured by measuring the change in absorbance at 450 nm.

Applications

It is suitable to detect the activity of NADP-malate dehydrogenase (NADP-MDH) in animal tissue, serum (plasma) and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue, serum (plasma) and cell samples

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